Isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel

Petroleum products are used for energy production and an essential part of our day-to-day lives especially in vehicles, ships, and industries. Accidental leakages occur easily and wastage petroleum is also discarded in the environment without any further processing causing environmental pollution. Diesel contributea big part topetroleum pollution. The current study was aimed to identify diesel degrading bacteria and determine some conditions to evaluate their best degradation capability. We identified Aeromonas spp., Bacillus spp., and Enterobacter spp. from diesel contaminated soil and found that Aeromonas spp. and Bacillus spp. grow best with 10% to 15% diesel whereas Enterobacter spp. can grow quite well with 20% diesel concentration at a higher temperature (40oC) than the previous two bacteria. Aeromonas spp. worked well at low pH (pH 4 to pH 6) whereas Bacillus spp. and Enterobacter spp. worked best at higher pH (pH 10).

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Isolation and characterization of a novel phenanthrene (PHE) degrading strain Psuedomonas sp. USTB-RU from petroleum contaminated soil

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2013.10.007 ◽

2013 ◽

Vol 263 ◽

pp. 493-500 ◽

Cited By ~ 33

Author(s):

Kanaji Masakorala ◽

Jun Yao ◽

Minmin Cai ◽

Radhika Chandankere ◽

Haiyan Yuan ◽

...

Keyword(s):

Contaminated Soil ◽

Isolation And Characterization ◽

Petroleum Contaminated Soil

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Isolation and characterization of diesel degrading bacteria, Sphingomonas sp. and Acinetobacter junii from petroleum contaminated soil

Frontiers of Earth Science ◽

10.1007/s11707-013-0415-6 ◽

2014 ◽

Vol 8 (1) ◽

pp. 58-63 ◽

Cited By ~ 8

Author(s):

Qiuzhuo Zhang ◽

Duanchao Wang ◽

Mengmeng Li ◽

Wei-Ning Xiang ◽

Varenyam Achal

Keyword(s):

Contaminated Soil ◽

Acinetobacter Junii ◽

Isolation And Characterization ◽

Degrading Bacteria ◽

Petroleum Contaminated Soil

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Isolation and Characterization of Hydrocarbon Degrading Bacteria from Petrol Contaminated Soil

Journal of Basic & Applied Sciences ◽

10.6000/1927-5129.2015.11.32 ◽

2015 ◽

Vol 11 ◽

pp. 223-231 ◽

Cited By ~ 6

Author(s):

Abdul Wahab ◽

◽

Talat Mujahid ◽

Safia Padhiar ◽

Syed Subhan ◽

...

Keyword(s):

Contaminated Soil ◽

Isolation And Characterization ◽

Degrading Bacteria

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Rhizosphere biodegradation studies on long-term PCB contaminated soil; isolation and characterization of different rhizosphere microbial communities from PCBs soil

Journal of Biotechnology ◽

10.1016/j.jbiotec.2007.07.431 ◽

2007 ◽

Vol 131 (2) ◽

pp. S236-S237 ◽

Cited By ~ 1

Author(s):

Marieta Ionescu ◽

Katarina Beranova ◽

Lucia Kochankova ◽

Katerina Demnerova ◽

Tomas Macek ◽

...

Keyword(s):

Microbial Communities ◽

Contaminated Soil ◽

Isolation And Characterization

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Isolation and characterization of a novel native Bacillus strain capable of degrading diesel fuel

International Journal of Environmental Science and Technology ◽

10.1007/bf03326082 ◽

2009 ◽

Vol 6 (3) ◽

pp. 435-442 ◽

Cited By ~ 52

Author(s):

D. Yousefi Kebria ◽

A. Khodadadi ◽

H. Ganjidoust ◽

A. Badkoubi ◽

M. A. Amoozegar

Keyword(s):

Diesel Fuel ◽

Bacillus Strain ◽

Isolation And Characterization

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Isolation and Characterization of Rhodopseudomonas sp. S9-1 Capable of Degrading Pyrazosulfuron-Ethyl

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.356-360.1152 ◽

2011 ◽

Vol 356-360 ◽

pp. 1152-1163 ◽

Cited By ~ 5

Author(s):

Le Bin Yin ◽

Yong Liu ◽

De Yong Zhang ◽

Song Bai Zhang

Keyword(s):

Contaminated Soil ◽

Acetolactate Synthase ◽

Optimal Growth ◽

Degradation Process ◽

Amino Acid Sequences ◽

Rrna Gene ◽

Photosynthetic Membrane ◽

Physiological And Biochemical Characteristics ◽

Isolation And Characterization

A bacterial strain S9-1capable of degrading sulfonylurea herbicide pyrazosulfuron-ethyl (PSE) was isolated from contaminated soil through the enrichment incubation method. Based on morphology, colony and cultural properties, physiological and biochemical characteristics, living-cell absorption spectra, internal photosynthetic membrane, and phylogenetics of its 16S rRNA gene sequence, S9-1was preliminarily identified as belonging to the genus Rhodopseudomonas, a group of photosynthetic bacteria (PSB). The effects of PSE concentration, pH, and temperature on biodegradation were examined. The degradation rate was found to decrease with increasing PSE concentration. Optimal growth pH and temperature were found to be 7.0 and 30°C, respectively. The strain was able to degrade 47.51% of PSE at a concentration of 100 mg ml-1after 7 days of incubation at 30°C and could tolerate 800 mg ml-1PSE. S9-1was also able to completely co-metabolically transform 100 mg ml-1PSE at 30°C, pH 7.0, and 7500 lux in 15 days. As the concentration of PSE increased, the degradation process took longer to complete. The fragment encoding acetolactate synthase (ALS) gene from S9-1was cloned and sequenced. Comparison of deduced amino acid sequences was implemented, and the conserved sites were analyzed. To our knowledge, this is the first report of PSB in PSE biodegradation. These results highlight the potential of this bacterium as a detoxifying agent for use with PSE-contaminated soil and wastewater.

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